Updated: Sep 26
Researchers from the University of Montréal hypothesized that prolonged exposure to two specific cannflavins, cannflavin A (Can A) and cannflavin B (Can B), could lead to desensitization of the vanilloid receptor. They aimed to investigate whether this desensitization would hinder the nocifensive (pain-avoidance) responses to noxious thermal stimuli.
To explore this further, the researchers conducted experiments using the nematode Caenorhabditis elegans. They exposed both wild-type (N2) and mutant nematodes to solutions containing Can A and Can B for a duration of 60 minutes. Afterwards, the nematodes were placed on Petri dishes divided into quadrants and subjected to thermal stimulation. The researchers then measured the thermal avoidance index for each group of C. elegans.
Through proteomics and Reactome pathway analyses, the researchers identified specific proteins and pathways associated with the treatment of Can A or Can B. Interestingly, both treatments were found to be related to eukaryotic translation initiation and metabolic processes strongly associated with pain development. This study contributes to our understanding of the pharmacological properties of cannflavins derived from Cannabis sativa. It suggests the potential application of these compounds as pain therapy.
We encourage interested readers to delve deeper into our research by reading the full paper at: https://link.springer.com/article/10.1007/s00210-023-02621-3